Mouse IgG1 Antibody Biotin Conjugated

SKU: orb347598

Description

Mouse IgG1 antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:250,000, IHC: 1:500 - 1:3,000, WB: 1:1,000 - 1:5,000
• Reactivity: Mouse
• Application Notes:
  Mouse IgG1 secondary antibody conjugated to Biotin is available in a variety of formats. Anti-Mouse IgG1 Biotin Antibody has been tested by ELISA and western blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Mouse IgG1 heavy chain
• Purity: Mouse IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA. Typically, less than 1% cross-reactivity was observed against other Mouse heavy chain isotypes.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody biotin Conjugation, Rabbit Anti-Mouse IgG1 BAC Conjugated Antibody

ELISA Results of Rabbit Anti-Mouse IgG1 Antibody Biotin Conjugation tested against purified Mouse IgG1 Biotin. Each well was coated in duplicate with 1.0 µg of Mouse IgG1 (p/n orb343776). The working dilution is 1:146000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Streptavidin-HRP conjugated (p/n orb348767) and TMB substrate (p/n orb348651).