Mouse IgG (H&L) Antibody

SKU: orb750705

Description

Mouse IgG (H&L)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Mouse
• Application Notes:
  Anti-MOUSE IgG (H&L) (GOAT) Antibody has been tested by western blot and is suitable for dot blot, ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Specific conditions should be optimized by user.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: Mouse IgG whole molecule
• Purity: Anti-MOUSE IgG (H&L) (GOAT) Antibody was prepared from monospecific antiserum by a delipidation and defibrination. Anti-MOUSE IgG (H&L) (GOAT) Antibody Assay by immunoelectrophoresis resulted in a single precipitin arc against Mouse IgG and Mouse Serum.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 85 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Goat Anti-Mouse Ab, goat anti-mouse IgG, Mouse Secondary Antibody, Mouse Antibody in Goat, Gt-a-Mouse Antibody

Western Blot of Goat Anti-Mouse IgG (H&L) Antibody. Lane M: Molecular Ladder. Lane 1: Mouse IgG whole molecule (p/n orb2652749). Lane 2: Mouse IgG F(c) Fragment (p/n orb346280). Lane 3: Mouse IgG F(ab) Fragment (p/n orb346282). Lane 4: Mouse IgM Kappa (p/n orb346283). Lane 5: Mouse Serum. Load: 50 ng per lane. Block: (p/n orb348637) for 30 min at RT. Primary Antibody: Anti-Mouse IgG (H&L) Antibody 1:1000 for 60 min at RT. Secondary antibody: HRP Donkey Anti-Goat IgG (p/n orb348124) 1:40000 for 30 min at RT. Predicted/Observed Size: 28 and 55 kDa for Mouse IgG, F(c), F(ab), IgM Kappa, and Serum. Mouse F(c) migrates slightly higher.