Mouse IgG (H&L) Antibody

SKU: orb347415

Description

Mouse IgG (H&L) antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:25,000, IHC: 1:1,000 - 1:5,000, WB: 1:3,000 - 1:15,000
• Reactivity: Mouse
• Application Notes:
  Anti-Mouse IgG affinity purified antibody is generated in goat detects specifically Mouse IgG whole molecule. This anti-Mouse IgG secondary antibody has been tested by ELISA and western blot and is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat.
• Purity: Secondary Antibody Anti-Mouse IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store Anti-Mouse Secondary Antibody at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Goat anti-Mouse IgG Secondary Antibody, Mouse Secondary Antibody, GAM Antibody, Gt-a-Ms antibody, anti-mouse secondary, Goat anti Mouse IgG

ELISA Results of Goat Anti-Mouse IgG Antibody tested against purified Mouse IgG protein. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n orb2652749). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1:35800. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Donkey Anti-Goat IgG Peroxidase Conjugate at 1:40000 and TMB substrate p/n orb348651.

Western Blot of Goat anti-Mouse IgG (H&L) Antibody. Lane 1: Mouse IgG. Load: 100 ng per lane. Primary Antibody: Mouse IgG (H&L) Antibody 1:1000 overnight at 4°C. Secondary antibody: DyLight™ 800 goat secondary antibody at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed Size: 28 and 55 kDa/28 and 55 kDa for Mouse IgG. Other band(s): none.

Western Blot of Goat Anti-Mouse IgG (H&L) Antibody. Lane M: Molecular Ladder. Lane 1: Mouse IgG whole molecule (p/n orb2652749). Lane 2: Mouse IgG F(c) Fragment (p/n orb346280). Lane 3: Mouse IgG F(ab) Fragment (orb346282). Lane 4: Mouse IgM Kappa (p/n orb343770). Lane 5: Mouse Serum. Load: 50 ng per lane. Block: (p/n orb348637) for 30 min at RT. Primary Antibody: Anti-Mouse IgG (H&L) Antibody 1:1000 for 60 min at RT. Secondary antibody: HRP Donkey Anti-Goat IgG (p/n orb347032) at 1:40000 for 30 min at RT. Predicted/Observed Size: 28 and 55 kDa for Mouse IgG, F(c), F(ab), IgM Kappa, and Serum. Mouse F(c) migrates slightly higher.