Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Peroxidase Conjugated

SKU: orb2652711

Description

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Peroxidase Conjugated

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:40,000, IHC: 1:1000 - 1:5000, WB: 1:2000 - 1:10000
• Reactivity: Mouse
• Application Notes:
  This Anti-Mouse IgG subclass pan reactive Peroxidase Antibody has been tested by ELISA and is designed for Western Blotting, ELISA and Immunohistochemistry. HRP conjugated secondary antibodies can also be used for a variety of other applications such as Assay Development. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 100 µL

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins
• Purity: Anti-Mouse IgG subclass pan reactive Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody peroxidase conjugation, rabbit anti-Mouse IgG HRP conjugated Antibody

Indirect ELISA of Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b, and 3) antibody. Antigen: purified mouse IgG heavy and light chains. Coating amount: 0.1 µg per well. Primary antibody: Rabbit Anti-Mouse IgG (Gamma 1, 2a, 2b, and 3) HRP conjugated Antibody. Dilution series: 2-fold. Substrate: TMB.