Mouse IgG Fc Antibody Pre-Adsorbed

SKU: orb347420

Description

Mouse IgG F(c) antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:450,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:50,000
• Reactivity: Mouse
• Application Notes:
  Anti-Mouse IgG F(c) antibody has been tested by ELISA and is suitable for ELISA, Western Blot and Immunohistochemistry applications. Antibody should be optimized by end user for specific reactive conditions.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Mouse IgG F(c) fragment
• Purity: Anti-Mouse IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab')2 or Bovine, Horse and Human Serum Proteins.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.20 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Immunoglobulin, Mouse IgG, Goat Anti-Mouse IgG F(c) Antibody, Goat Anti-Mouse IgG Fc Antibody, Goat Anti-Mouse IgG Fc Fragment Antibody

ELISA Results of purified Goat Anti-Mouse IgG F(c) Min X Bovine Horse and Human Serums. Each well was coated in duplicate with 1.0 µg of Mouse IgG (orb2652749). The working dilution is 27300. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Dky Anti- Goat IgG HRP conjugated (p/n orb347032) and TMB substrate (p/n orb348651).