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Mouse IgG Fc Antibody Peroxidase Conjugated

SKU: orb346670

Description

Mouse IgG F(c) antibody (Peroxidase)

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
ReactivityMouse
Application Notes
Mouse IgG F(c) Antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Key Properties

Antibody TypeSecondary Antibody
HostGoat
ClonalityPolyclonal
IsotypeIgG
ImmunogenAnti-Mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in goat.
PurityThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab).
ConjugationHRP

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/PreservativesPreservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration10.0 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Mouse IgG F(c) Antibody, Gt-a-Mouse F(c), Mouse F(c) Antibody in goat, Mouse F(c) Secondary Antibody, goat anti-Mouse IgG F(c) fragment Peroxidase Conjugated Antibody

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Mouse IgG Fc Antibody Peroxidase Conjugated (orb346670)

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20 mg
$ 710.00
DispatchUsually dispatched within 5-10 working days
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