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Catalog Number | orb257942 |
---|---|
Category | Proteins |
Description | Mouse Dkk-1, His Tag is expressed from human 293 cells (HEK293). It contains AA Thr 32 - His 272 (Accession # O54908). |
Target | Dkk-1 |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Mouse |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | Thr 32 - His 272 |
UniProt ID | O54908 |
MW | 28.0 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 28.0 kDa. The protein migrates as 41-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Mouse |
Expression Region | Thr 32 - His 272 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
SDS-PAGE analysis of Mouse Dkk-1 protein
Greater than 95% as determined by SDS-PAGE. | |
HEK293 Cells |
> 95%, determined by SDS-PAGE | |
This protein contains the mouse DKK1 (NP_0341812) (Met1-His272) was expressed with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |
> 95%, determined by SDS-PAGE | |
This protein contains the mouse DKK1 (NP_0341812) (Met1-His272) was expressed with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |
> 87% by SDS-PAGE. | |
Recombinant Mouse Dkk-1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met1-His Tag272) of mouse Dkk-1 (Accession #NP_034181.2. ) fused with a 6��His Tag at the C-terminus. |