Mouse CIP2A Monoclonal Antibody

SKU: orb1520149

Description

CIP2A Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:1000; IP - 20 µl/mg lysate
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: 2A1-9A4
• Immunogen: Amino terminal one-third of the CIP2A (GeneID 57650) recombinant protein
• Target: CIP2A
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 40 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cancerous inhibitor of PP2A; cancerous inhibitor of protein phosphatase 2A; KIAA1524; p90; p90 autoantigen; protein CIP2A

Detection of human CIP2A by western blot. Samples: Whole cell lysate (25 µg) from HEK293T, Jurkat, U2OS, HeLa, and A-549 cells prepared using NETN lysis buffer. Antibody: Mouse anti-CIP2A monoclonal antibody [2A1-9A4] (orb1520149) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Detection of mouse CIP2A by western blot. Samples: Whole cell lysate (50 µg) from BW5147.3, CT26, Ba/F3, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Mouse anti-CIP2A monoclonal antibody [2A1-9A4] (orb1520149) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human CIP2A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Mouse anti-CIP2A monoclonal antibody [2A1-9A4] (orb1520149) used for IP at 20 µl/mg lysate.

Quick Database Links

Gene Symbol

CIP2A

UniProt

Q8TCG1

RefSeq (Protein):NP_065941.1