Mono-Methyl-Histone H4 (Arg17) Rabbit Polyclonal Antibody

SKU: orb1172872

Description

Mono-Methyl-Histone H4 (Arg17) Rabbit Polyclonal Antibody

Research Area

DNA and RNA, Epigenetics

Images & Validation

• Tested Applications: ChIP, WB
• Dilution range: WB=1:500-2000, ChIP=6 μg/5x106 cells
• Reactivity: Human, Monkey, Mouse, Plant, Rat
• Predicted Reactivity: Monkey, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Target: H4C1
• Molecular Weight: 11 kDa
• Purification: Antigen affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: HeLa, BRL, N2a, COS-7, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 11 kDa, Observed MW: 11 kDa.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: Mouse liver, Mouse spleen, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 11 kDa, Observed MW: 11 kDa.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: Rice, Arabidopsis, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 11 kDa, Observed MW: 11 kDa.

Quick Database Links

Gene Symbol

H4C1