MMP3 Antibody (Center)

SKU: orb1930181

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB02015-02016
• Target: This MMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-327 amino acids from the Central region of human MMP3.
• Molecular Weight: 53977 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

Stromelysin-1, SL-1, Matrix metalloproteinase-3, MMP-3, Transin-1, MMP3, STMY1

Flow cytometric analysis of HepG2 cells using MMP3 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MMP3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-MMP3 Antibody (H313) at 1:1000 dilution. Lane 1: U118MG whole cell lysate. Lane 2: U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

This MMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-327 amino acids from the Central region of human MMP3.

UniProt

P08254

RefSeq (Protein):NP_002413.1