Mitochondrial Antibody [MTC719]

SKU: orb1252655

Description

Mitochondrial Antibody [MTC719]

Research Area

Metabolism Research

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 µg/mLWB: 0.25-0.5 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Mitochondrial antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: MTC719
• Immunogen: The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondrial antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human bladder carcinoma with Mitochondrial antibody.

IHC testing of FFPE human renal cell carcinoma with Mitochondrial antibody.

IHC testing of FFPE human pancreas with Mitochondrial antibody.