Mito-TEMPO

SKU: orb1307030

Description

Mito-TEMPO is a mitochondria-targeted antioxidant that acts as a superoxide dismutase mimetic, effectively scavenging superoxide and alkyl radicals. Its application in both in vitro and in vivo research is crucial for studying and mitigating oxidative stress-induced mitochondrial dysfunction, apoptosis, and necrotic cell death.

Research Area

Immunology & Inflammation, Metabolism Research, Signal Transduction

Key Properties

• CAS Number: 1334850-99-5
• MW: 510.03
• Purity: 99.12% (May vary between batches)
• Formula: C₂₉H₃₅N₂O₂P.Cl
• SMILES: [O]N1C(C)(C)CC(NC(C[P+](C2=CC=CC=C2)(C3=CC=CC=C3)C4=CC=CC=C4)=O)CC1(C)C.[Cl-]
• Target: Mitochondrial Metabolism,ROS,Reactive Oxygen Species
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (9.8 mM);H2O:60 mg/mL (117.64 mM);DMSO:255 mg/mL (499.97 mM)

Bioactivity

• In Vivo:
  METHODS: To investigate the protective effect against hepatotoxicity, APAP (300 mg/kg) was intraperitoneally injected into C57BL/6J mice, and Mito-TEMPO (20 mg/kg in saline) was injected intraperitoneally 1.5-3 h later. RESULTS: Mito-TEMPO had a protective effect on the late hepatotoxicity of APAP. METHODS: To investigate the effects on coronary vasodilatation and endothelial SK channel activity, Mito-TEMPO (1 mg/kg in saline) was intraperitoneally injected into C57BL/6J mice with or without diabetes once daily for four weeks. RESULTS: After 4 weeks of treatment with Mito-TEMPO, diabetic mice showed significantly improved endothelium-dependent diastolic responses of coronary arteries to ADP or NS309 and endothelial SK channel currents compared to untreated diabetic mice.
• In Vitro:
  METHODS: Human neuroblastoma cells SH-SY5Y were treated with Mito-TEMPO (25-100 μM) for 24 h. Cell viability was detected using MTT assay. RESULTS: No cytotoxic effect was shown on the cells in the Mito-TEMPO-treated group, and a significant increase in cell viability was detected after Mito-TEMPO treatment. METHODS: Normal rat proximal renal tubular epithelial cell line NRK-52E was pretreated with Mito-TEMPO (10 μM) for 1 h, then stimulated with oxalate (700 μM) for 1 h. The mitochondrial membrane potential was detected by using MMP assay kit (JC-1). RESULTS: The control cells showed bright red fluorescence. Compared with the control, oxalate treatment attenuated the red fluorescence, and these changes were reversed by pretreatment with Mito-TEMPO. The RESULTS suggest that oxalate induces mitochondrial dysfunction, and Mito-TEMPO can inhibit this effect.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Reactive Oxygen Species, ReactiveOxygenSpecies, Inhibitor, inhibit, Mitochondrial Metabolism, MitochondrialMetabolism, Mito TEMPO, MitoTEMPO, Mito-TEMPO

Compound Identifiers

PubChem CID

134828258