CCL20 Antibody (Biotin)

SKU: orb345177

Description

MIP-3 alpha antibody (Biotin)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
• Reactivity: Human
• Application Notes:
  This purified antibody has been tested in western blotting and is suitable in ELISA. By western blot a band approximately 11 kDa in size corresponding to human MIP-3α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: MIP-3 alpha Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-3α protein.
• Target: CCL20
• Purity: Human MIP-3 alpha Antibody Biotin Conjugated was heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human MIP-3α in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
• Conjugation: Biotin

Storage & Handling

• Storage: Store MIP-3 alpha Antibody Biotin Conjugated at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-MIP-3 alpha Antibody Biotin Conjugated, rabbit anti-MIP-3a biotin Conjugated Antibody, MIP-3α , CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1

Western Blot of Biotin Conjugated Rabbit Anti-Human MIP-3α Antibody. Lane 1: Human MIP-3α. Lane 2: None. Load: 50 ng per lane. Primary antibody: Human MIP-3α antibody at 1:1000 for 60 min at RT. Secondary antibody: Peroxidase conjugated Streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 10 kDa, 10 kDa for Human MIP-3α. Other band(s): None.

Quick Database Links

Gene Symbol

CCL20

UniProt

P78556

RefSeq:4759076