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Catalog Number | orb1539378 |
---|---|
Category | Antibodies |
Description | MICU1/CBARA1 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, IHC-P, WB |
Reactivity | Bovine, Canine, Equine, Gallus, Goat, Hamster, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat, Xenopus, Zebrafish |
Immunogen | Peptide with sequence C-EYENRIRAYSTPDK, from the internal region of the protein sequence according to NP_006068.2NP_001182447.1. |
Concentration | 0.5 mg/ml |
Dilution range | IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:32000), WB (0.5 - 2 µg/ml) |
Conjugation | Unconjugated |
Target | MICU1 / CBARA1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin |
Alternative names | MICU1, Ara CALC, CBARA1, CALC, EFHA3, Mitochondria Read more... |
Note | For research use only |
Application notes | Further information: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 55kD band observed in lysates of cell line K562 (calculated MW of 54.6kD according to NP_006068.2). Recommended concentration: 0.5-2 ug/ml. An additional band of unknown identity was also consistently observed at 37kD. This band was successfully blocked by incubation with the immunizing peptide. |
Expiration Date | 12 months from date of receipt. |
CBARA1 antibody (0.5 ug/ml) staining of K562 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.