Methyl pyropheophorbide-a

SKU: orb2901150

Description

Methyl pyropheophorbide-a (Pyropheophorbide-a methyl ester), a chlorophyll-a derivative, is a potent photosensitizer that can be used in photodynamic therapy (PDT) of cancer. Methyl pyropheophorbide-a has photodynamic activity and can induce apoptosis and inhibit tumor growth.

Key Properties

• CAS Number: 6453-67-4
• MW: 548.67
• Purity: >98% (HPLC)
• Formula: C₃₄H₃₆N₄O₃
• SMILES: CCc1c(C)c2cc3[nH]c(cc4nc([C@@H](CCC(=O)OC)[C@@H]4C)c4CC(=O)c5c(C)c(cc1n2)[nH]c45)c(C)c3C=C
• Target: Apoptosis
• Solubility: In Vitro: DMSO : 2.5 mg/mL (4.56 mM; Ultrasonic (<60°C)

Bioactivity

• In Vivo:
  Pyropheophorbide-a methyl ester (15 mg/kg; tail vein and topical injection) inhibits the growth of PC-3M tumors in mice. Animal model: Male BALB/c nude mice (6-8 weeks, 16-20 g) bearing PC-3M tumor. Dosage: 15 mg/kg. Administration: Tail vein injection and topical injection. Result: The tumor volume and the weight inhibition rate were 46.78%, 41.84% in tail vein injection group and 78.66%, 72.07% in topical injection group, respectively.
• In Vitro:
  Pyropheophorbide-a methyl ester (2 μM; 12 h) inhibits the cell cycle progression from the G0/G1-phases and induces apoptosis at the light dosage of 55.6 kJ/m2 in PC-3M cells. Pyropheophorbide-a methyl ester (0.2-15 μM; 20 h) has no signi cant dark cytotoxicity in NCI-h446 cells. Pyropheophorbide-a methyl ester (0.1-15 μM; 20 h) mediates a dose-dependent photocytotoxicity in the NCI-h446 cells. Pyropheophorbide-a methyl ester (2-4 μM; 0-24 h) induces apoptosis of NCI-h446 cells in a time-dependent manner. Pyropheophorbide-a methyl ester (0.5-4 μM; 0-20 h) shows a photocytotoxicity in PC-3M cells. Apoptosis Analysis Cell line: PC-3M cells. Concentration: 2 μM. Incubation time: 12 h. Result: Apoptosis rate was about 0.27%, 6.15%, 20.49%, 50.76%, 65.39%, 64.92%, and 64.37% at 1, 3, 6, 12, 18, and 24 h posttreatment, respectively under the conditions of LC75 (2 μM+55.6 kJ/m2).

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Apoptosis