MDC1 Antibody

• Catalog Number: orb67385
• Category: Antibodies
• Description: Mouse Anti-Mouse MDC1 Monoclonal IgG1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: P2B11
• Tested applications: ICC, IF, WB
• Reactivity: Bovine, Human, Mouse, Primate
• Isotype: IgG1
• Immunogen: GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus
• Concentration: 1 mg/ml
• Dilution range: WB (1:2000), ICC/IF (1:100)
• Conjugation: Unconjugated
• MW: 184kDa
• Target: MDC1
• Entrez: 240087
• UniProt ID: Q5PSV9
• NCBI: NP_001010833.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Alternative names: Nuclear factor with BRCT domains1 Antibody, mediat
• Note: For research use only
• Application notes: 0.5 µg/ml of SMC-197 was sufficient for detection of MDC1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.

Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11. Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP: IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.

Western Blot analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11. Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.