MAPK14 Antibody (Center T180/Y182)

• Catalog Number: orb1937427
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This MAPK14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-192 amino acids from the Central region of human MAPK14.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat, Zebrafish
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q16539
• MW: 41293 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB30167
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Mitogen-activated protein kinase 14, MAP kinase 14
• Research Area: Cancer Biology, Cell Biology, Immunology & Inflamm
• Note: For research use only
• NCBI: NP_001306.1, NP_620581.1, NP_620583.1, NP_620582.1
• Expiration Date: 12 months from date of receipt.

Fluorescent confocal image of A549 cell stained with MAPK14 Antibody (Center T180/Y182). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MAPK14 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). MAPK14 immunoreactivity is localized to Vesicles significantly and Cytoplasm weakly.

MAPK14 Antibody (Center T180/Y182) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MAPK14 Antibody (Center T180/Y182) for immunohistochemistry. Clinical relevance has not been evaluated.

MAPK14 Antibody (Center T180/Y182) western blot analysis in Jurkat cell line lysates (35 ug/lane). This demonstrates the MAPK14 antibody detected the MAPK14 protein (arrow).