MAP2 Antibody

SKU: orb1925242

Description

MAP2 Antibody

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50
• Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 199526 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot analysis of MAP2 Antibody in MCF-7 cell line lysates (35 ug/lane). MAP2 (arrow) was detected using the purified Pab.

MAP2 Antibody FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-MAP2 Antibody at 1:1000 dilution+ U-87 MG Cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 280kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded mouse brain tissue reacted with MAP2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

UniProt

P11137