Malachite green

SKU: orb1306779

Description

Malachite green

Key Properties

• CAS Number: 569-64-2
• MW: 364.91
• Purity: 97.75%
• Formula: C₂₃H₂₅ClN₂
• SMILES: [Cl-].CN(C)c1ccc(cc1)C(=C1C=CC(C=C1)=[N+](C)C)c1ccccc1
• Target: Others
• Solubility: DMSO:30 mg/mL (82.21 mM)

Bioactivity

• Cell Research:
  Instructions I. Solution preparation: 1. Preparation of storage solution: Dissolve Safranin O in deionized water to prepare a 0.1%-1% (w/v) stock solution. If a higher solubility is required, a small amount of ethanol can be added to help dissolve. Note: The stock solution should be stored in a dark place and refrigerated to avoid dye degradation. 2. Preparation of working solution: Dilute the storage solution to the required working solution according to experimental requirements. II. Biological tissue staining 1. Sample fixation: Fix the tissue sample in formalin solution, dehydrate and embed. After slicing, prepare the tissue slices for staining. 2. Staining steps: 1) Place the tissue slices in Safranin O staining solution. The staining time is adjusted according to the sample type, generally 2-5 minutes. 2) If contrast staining is required, Fast Green or other suitable dyes can be used. 3) Use 70%-95% ethanol for graded decolorization until the dye in non-target areas is removed. 4) Sealing: Seal the slide with neutral gum or other suitable sealing agent to prevent the sample from drying or dye loss. 5) Microscopic observation: Use an optical microscope to observe the staining results: Cell nuclei and cartilage matrix will appear red. Collagen fibers, extracellular matrix, etc. will appear green (if Fast Green is used as a contrast dye). Precautions: 1) Operation safety: Wear gloves and goggles when using to avoid contact of dye with skin or eyes. 2) Dye storage: Safranin O is light-sensitive and should be stored in a dark place and refrigerated. Ensure that there is no precipitation in the dye before use. 3) Contrast staining: Select a suitable contrast stain (such as Fast Green) according to the purpose of the experiment.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Malachite green, Solid Green Crystals O, Solid Green O

Compound Identifiers

PubChem CID

11294