M-31850

SKU: orb1218228

Description

M-31850 is a potent, selective and competitive β-hexosaminidase (Hex) inhibitor(human HexA and human HexB with IC50s of 6.0 μM and 3.1 μM, respectively). IT also competitively inhibits β-N-acetyl-D-hexosaminidase OfHex2 with a Ki of 2.5 μM.(In Vitro):M-31850 shows some activity towards Jack Bean Hex (JBHex) and bacterial Hex from Streptomyces plicatus (SpHex) (IC50 of 280 μM and >500 μM for JBHex and SpHex, respectively).M-31850 produces a dose dependent increase in MUG hydrolysis (Hex S levels) in lysates from treated infantile Sandhoff disease (ISD) cells.M-31850 increases the half-life of the mutant Hex A from Adult forms of Tay-Sachs (ATSD) cells more than two-fold at 44° C, relative to the enzyme heated in the presence of DMSO. M-31850 acts as a classic competitive inhibitor of Hex (Km increases and Vmax is unaffected by increasing amounts of M-31850), with a Ki of 0.8 μM.

Key Properties

• CAS Number: 281224-40-6
• MW: 463.48
• Purity: >98% (HPLC)
• Formula: C₂₈H₂₁N₃O₄
• SMILES: C1=CC2=C3C(=C1)C(=O)N(C(=O)C3=CC=C2)CCNCCN4C(=O)C5=CC=CC6=C5C(=CC=C6)C4=O
• Target: β-hexosaminidase (Hex)
• Solubility: DMSO:5 mg/mL (10.79 mM; ultrasonic and adjust pH to 6 with HCl)

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

1H-Benz[de]isoquinoline-1,3(2H)-dione, 2,2'-(iminodi-2,1-ethanediyl)bis-

Quick Database Links

Gene Symbol

β-hexosaminidase (Hex)