LIM Kinase antibody

• Catalog Number: orb345493
• Category: Antibodies
• Description: LIM Kinase antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal region near aa 620-647 of human LIM kinase protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:8,000 - 1:36,000, IF: User Optimized, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against human LIM kinase protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and orangutan based on 100% homology for the immunogen sequence. Expect cross reactivity with LIM kinase from mouse and rat sources as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). Cross reactivity with LIM kinase homologues from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P53667
• NCBI: 4505001
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-LIM Kinase antibody, LIMK-1, LIM domai
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 73 kDa in size corresponding to LIM kinase by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of mouse brain tissue using LIM Kinase antibody