Leptin Receptor (LEPR) / Ob-R / CD295 Antibody

• Catalog Number: orb750863
• Category: Antibodies
• Description: Mouse monoclonal antibody to Leptin Receptor
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LEPR/4301
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Recombinant fragment (around aa335-425) of human LEPR protein (exact sequence is proprietary)
• Concentration: 200 μg/ml
• Dilution range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 100kDa / 125kDa (short / long form)
• Target: LEPR
• Entrez: 3953
• UniProt ID: P48357
• Expression System: CHO cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Anti-Db antibody, anti-Fa antibody, anti-HuB219 an
• Note: For research use only
• Application notes: Positive Control: HepG2 or HeLa cells. Human heart, small intestine or fetal liver.
• Expiration Date: 12 months from date of receipt.