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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus LDL R, His Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human PCSK9, His Tag with a linear range of 0.007-0.256 ug/mL. |
| Target | LDL R |
| Tag | His Tag |
| MW | 86.8 kDa |
| Purity | >95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
| Protein Sequence | Ala 22 - Gly 788 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.3 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human LDL R Protein (Biotin) [orb2994702]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 88.4 KDa. Observed: 95-140 KDa, reducing conditions
20 μg, 100 μgRecombinant Human LDL R Protein [orb2994788]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 86.56 KDa. Observed: 99-135 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgLDL R Recombinant Protein [orb1227676]
ELISA, WB
>95% as determined by reduced SDS-PAGE.Less than 1.0 EU per ug by the LAL method.
86.2 kDa
HEK293 cells
0.1 mg

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Immobilized Cynomolgus LDL R, His Tag at 2 ug/ml (100 uL/well) can bind Biotinylated Human PCSK9, His Tag with a linear range of 0.007-0.256 ug/ml.
Quick Database Links
NCBI Reference Sequences
−| RefSeq | XP_005588053.1 |
|---|
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Protocol Information
LDL R Recombinant Protein (orb1227811)
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