LAR/PTPRF Antibody: PerCP

• Catalog Number: orb167667
• Category: Antibodies
• Description: Mouse monoclonal antibody to PTPRF (PerCP). PTPRF or leukocyte common antigen-related protein (LAR) is a widely expressed protein tyrosine phosphatase with an extracellular receptor region that resembles a cell adhesion molecule. PTPRF removes phosphate group from-catenin, an event that may subsequently facilitate cell-cell adhesion and ensure the stability of the cadherin complex. This phosphatase has also been implicated in various cellular processes such as neurite growth, nerve regeneration, actin remodeling and regulation of insulin function . Anti-PTPRF (C-terminal) antibody is specific for the extracellular and cytoplasmic subunits of human PTPRF (approx. 210, 150 and 85 kDa). Detection of the PTPRF bands by immunoblotting is specifically inhibited by the immunizing peptide.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N165/38 (Formerly sold as S165-38)
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2a
• Immunogen: Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR. 97% identical in both rat and mouse. > 80% identity with PTPRD and PTPRS. > 50% identity with PTPRM and PTPRK.
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000)
• Conjugation: PerCP
• MW: 85kDa
• Target: LAR
• Entrez: 5792
• UniProt ID: P10586
• NCBI: NP_002831.2
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Alternative names: FLJ43335 antibody, FLJ45062 antibody, FLJ45567 ant
• Note: For research use only
• Application notes: 1 µg/ml of SMC-443 was sufficient for detection of LAR/PTPRF in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LAR/PTPRF Monoclonal Antibody, Clone N165/38. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-LAR/PTPRF Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LAR/PTPRF Antibody (D) Composite.

Western Blot analysis of Rat Brain Membrane showing detection of LAR protein using Mouse Anti-LAR Monoclonal Antibody, Clone N165/38. Primary Antibody: Mouse Anti-LAR Monoclonal Antibody at 1:250.