LAPTM5 Antibody (N-term)

• Catalog Number: orb1939181
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB27746
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 29937 Da
• Target: This LAPTM5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-49 amino acids from the N-terminal region of human LAPTM5.
• UniProt ID: Q13571
• NCBI: NP_006753.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Lysosomal-associated transmembrane protein 5, Lyso
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-LAPTM5 Antibody (N-term) at 1:1000 dilution. Lane 1: U266B1 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 30 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

LAPTM5 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

LAPTM5 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph node followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LAPTM5 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.