Koumine

SKU: orb1222734

Description

Koumine is an indole alkaloid. It reverses thermal hyperalgesia and mechanical allodynia induced by sciatic nerve chronic constriction injury (CCI) in rats in a dose-dependent manner. This effect is mediated by elevated allopregnanolone levels in the spinal cord.

Key Properties

• CAS Number: 1358-76-5
• MW: 306.4
• Purity: >98% (HPLC)
• Formula: C₂₀H₂₂N₂O
• SMILES: CN1CC2(C3CC4C5=NC6=CC=CC=C6C52CC1C3CO4)C=C
• Target: Others
• Solubility: DMSO : 31.25 mg/mL 101.99 mM

Bioactivity

• In Vivo:
  Koumine is less toxic, with the median lethal dose (LD50) of 300.0 mg/kg on Wistar rats. Koumine (0.6, 3, or 15 mg/kg/per, p.o.) exhibits antirheumatic properties in rats with adjuvant-induced arthritis (AIA) and collagen-induced arthritis (CIA). Koumine inhibits the increase in cytokines in joint tissue and TNF-α level in serum at 15 mg/kg, and suppresses the increase in the serum level of IL-1β at 3 and 15 mg/kg. Koumine (0.28, 7 mg/kg, s. c.) significantly reduces neuropathic pain after nerve injury. Koumine suppresses the increased Iba-1 protein level.
• In Vitro:
  Koumine (0.5, 1 and 2 mg/mL) dose- and time-dependently inhibits the proliferation of MCF-7 cells, with an IC50 of 124 μg/mL at 72 h. Koumine induces apoptosis, causes cell cycle arrest at G2/M phase. Koumine (0.5, 1 and 2 mg/mL) up-regulates the Bax/Bcl-2 ratio and caspase-3 expression in a dose-dependent manner in MCF-7 Cells. Koumine (25, 50, 100, and 200 μM) decreases the protein and mRNA levels of microglia M1 polarization factors in LPS-induced BV2 cells.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Koumine

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