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Catalog Number | orb1928387 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB21885 |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Dilution range | IF: 1:25, WB: 1:2000, WB: 1:1000, WB: 1:2000, IHC-P: 1:50~100, FC: 1:25 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 26314 Da |
Target | This KLRC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-206 amino acids from the C-terminal region of human KLRC1. |
UniProt ID | P26715 |
NCBI | NP_015567.1, NP_002250.1, NP_998822.1, NP_998823.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | NKG2-A/NKG2-B type II integral membrane protein, C Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of KLRC1 Antibody (C-term) in Jurkat cell line lysates (35 ug/lane). KLRC1 (arrow) was detected using the purified Pab.
Anti-KLRC1 Antibody (C-term) at 1:2000 dilution + Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human kidney carcinoma with KLRC1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes: Anti-KLRC1 Antibody (C-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: K562 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling KLRC1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
Overlay histogram showing Jurkat cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
FC, IF, IHC-P, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |