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KLRC1 Antibody (C-term)

Catalog Number: orb1928387

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1928387
CategoryAntibodies
DescriptionAffinity Purified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB21885
Tested applicationsFC, IF, IHC-P, WB
ReactivityHuman
IsotypeRabbit IgG
Dilution rangeIF: 1:25, WB: 1:2000, WB: 1:1000, WB: 1:2000, IHC-P: 1:50~100, FC: 1:25
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
ConjugationUnconjugated
MW26314 Da
TargetThis KLRC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-206 amino acids from the C-terminal region of human KLRC1.
UniProt IDP26715
NCBINP_015567.1, NP_002250.1, NP_998822.1, NP_998823.1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesNKG2-A/NKG2-B type II integral membrane protein, C
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NoteFor research use only
Expiration Date12 months from date of receipt.
KLRC1 Antibody (C-term)

Western blot analysis of KLRC1 Antibody (C-term) in Jurkat cell line lysates (35 ug/lane). KLRC1 (arrow) was detected using the purified Pab.

KLRC1 Antibody (C-term)

Anti-KLRC1 Antibody (C-term) at 1:2000 dilution + Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

KLRC1 Antibody (C-term)

Formalin-fixed and paraffin-embedded human kidney carcinoma with KLRC1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

KLRC1 Antibody (C-term)

All lanes: Anti-KLRC1 Antibody (C-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: K562 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

KLRC1 Antibody (C-term)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling KLRC1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

KLRC1 Antibody (C-term)

Overlay histogram showing Jurkat cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

  • KLRC1 Antibody (C-term) [orb1166198]

    FC,  IF,  IHC-P,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 30 μl