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Catalog Number | orb345438 |
---|---|
Category | Antibodies |
Description | KLH antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Mollusc |
Isotype | IgG |
Immunogen | KLH (Keyhole Limpet Hemocyanin) |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-KLH antibody was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and KLH. |
Conjugation | Unconjugated |
Storage | Store KLH antibody at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit Anti-KLH antibody, Keyhole Limpet Hemocyani Read more... |
Note | For research use only |
Application notes | Anti-KLH Antibody has been tested for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of KLH. Load: 10 ug using KLH antibody
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