KLH Antibody

SKU: orb345438

Description

KLH antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Reactivity: Other
• Application Notes:
  Anti-KLH Antibody has been tested for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH (Keyhole Limpet Hemocyanin)
• Purity: Anti-KLH antibody was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and KLH.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store KLH antibody at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit Anti-KLH antibody, Keyhole Limpet Hemocyanin

Western Blot of Rabbit anti-KLH antibody. Lane 1: Molecular Weight Marker. Lane 2: KLH. Load: 10 µg. Primary antibody: Anti-KLH at 1:1000 in 1% BSA-TBS, overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40000 for 45 min at RT. Block: 1% BSA-TTBS for 30 min RT. Predicted/Observed size: ~350-390 kDa for KLH.