Kir6.1 Antibody (Biotin)

SKU: orb376929

Description

Mouse monoclonal antibody against Kir6.1 conjugated to Biotin.

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:100 dilution of SMC-491 was sufficient for detection of Kir6.1 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2A
• Clone No.: N366/60 (Formerly sold as S366-60)
• Immunogen: Fusion protein amino acids 306-424 (Cytoplasmic C-terminus) of rat Kir6.1
• Target: Kir6.1
• Molecular Weight: 45kDa
• Purification: Protein G Purified

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Kir6.1, KCNJ8, Kcnj8, Inward rectifier K(+) channel Kir6.1, Potassium channel inwardly rectifying subfamily J member 8, ATP-sensitive inward rectifier potassium channel 8, uKATP-1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone N366/60. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kir6.1 Antibody (D) Composite.

Western Blot analysis of Rat Brain showing detection of ~45 kDa Kir6.1 protein using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone N366/60. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 μg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~45 kDa. Other Band (s): ~100 kDa.

Quick Database Links

Gene Symbol

Kir6.1

UniProt

Q63664

NCBI Gene ID

25472

RefSeq (Protein):NP_058795.3