Kir6.1 Antibody: Biotin

• Catalog Number: orb376929
• Category: Antibodies
• Description: Mouse monoclonal antibody against Kir6.1 conjugated to Biotin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N366/60 (Formerly sold as S366-60)
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2A
• Immunogen: Fusion protein amino acids 306-424 (Cytoplasmic C-terminus) of rat Kir6.1
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Conjugation: Biotin
• MW: 45kDa
• Target: Kir6.1
• Entrez: 25472
• UniProt ID: Q63664
• NCBI: NP_058795.3
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: ATP-sensitive inward rectifier potassium channel 8
• Note: For research use only
• Application notes: A 1:100 dilution of SMC-491 was sufficient for detection of Kir6.1 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone N366/60. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kir6.1 Antibody (D) Composite.

Western Blot analysis of Rat Brain showing detection of ~45 kDa Kir6.1 protein using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone N366/60. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 μg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~45 kDa. Other Band (s): ~100 kDa.