KIF5B Antibody

SKU: orb1247955

Description

KIF5B Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 110kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110kDa according to NP_004512.1). Recommended concentration: 0.5-1.5µg/mL.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QPVAVRGGGGKQV
• Target: KIF5B
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KIF5B, UKHC, kinesin 1 (110-120kD), kinesin family member 5B, KNS, KINH, KNS1, U-KHC, kinesin heavy chain

orb1247955 (1 ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. This data is from a previous batch, not on sale.

orb1247955 (2.5 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247955 (0.03 ug/ml) staining of lysates of cell line HeLa (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

KIF5B

UniProt

P33176

RefSeq (Protein):NP_004512.1