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Description
Research Area
Metabolism Research
Images & Validation
−Item 1 of 1
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein of human KDM5A |
| Target | KDM5A |
| Molecular Weight | Observed: 192kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−RBBP-2, RBBP2, RBP2, lysine-specific demethylase 5A, Jumonji, AT rich interactive domain 1A (RBP2-like), histone demethylase JARID1A, jumonji/ARID domain-containing protein 1A, lysine (K)-specific demethylase 5A, retinoblastoma binding protein 2
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Western blot analysis of extracts of various cell lines, using KDM5A antibody (orb1256513) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Quick Database Links
Gene Symbol
KDM5A
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
KDM5A Antibody (orb1256513)
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