Josephin-2 Rabbit Polyclonal Antibody

SKU: orb184482

Description

Josephin-2 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1:100-500, ELISA=1:5000-10000
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Josephin-2 (91-188/188aa)
• Target: JOSD2
• Molecular Weight: 21 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

FLJ29018; JOS2_HUMAN; JOSD 2; JOSD2; Josephin 2; Josephin domain containing 2; Josephin domain containing protein 2; Josephin domain-containing protein 2; Josephin-2; Josephin2; SBBI54.

Sample: Lane 1: Human MCF-7 cell lysates, Lane 2: Human U251 cell lysates, Lane 3: Human HT-1080 cell lysates, Lane 4: Human A431 cell lysates, Primary: Anti-Josephin-2 (orb184482) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kDa, Observed band size: 15, 21 kDa.

Quick Database Links

Gene Symbol

JOSD2

UniProt

Q8TAC2

References

Tao Yuan # 1, Chenming Zeng # 2, Jiawei Liu # 3, Chenxi Zhao 1, Fujing Ge 1, Yuekang Li 1, Meijia Qian 1, Jiamin Du 1, Weihua Wang 1, Yonghao Li 1, Yue Liu 1, Xiaoyang Dai 4, Jianya Zhou 5, Xueqin Chen 6, Shenglin Ma 6, Hong Zhu 7 8, Qiaojun He 9 10, Bo Yang Josephin domain containing 2 (JOSD2) promotes lung cancer by inhibiting LKB1 (Liver kinase B1) activity Signal Transduct Target Ther, 9, 11 (2024)

PubMed: 38177135

Applications

WB

Reactivity

Human

Xin Liu a b c † , Yi Fang c † , Mincong Huang d , Shiliang Tu b , Boan Zheng b , Hang Yuan b , Peng Yu b , Mengyao Lan d , Wu Luo c , Yongqiang Zhou e , Guorong Chen f , Zhe Shen g , Yi Wang c , Guang Liang Deubiquitinase JOSD2 alleviates colitis by inhibiting inflammation via deubiquitination of IMPDH2 in macrophages Acta Pharmaceutica Sinica B, (2024)

PubMed:

Applications

IF

Reactivity

Mouse

Si-Rui Shen 1 2, Zhu-Qi Huang 1 2 3, Yu-Die Yang 1 2, Ji-Bo Han 2 4, Zi-Min Fang 1 2, Yue Guan 1 2, Jia-Chen Xu 1 2, Ju-Lian Min 5, Yi Wang 2, Gao-Jun Wu 1, Zhong-Xiang Xiao 6, Wu Luo 7 8, Zhou-Qing Huang 9, Guang Liang JOSD2 inhibits angiotensin II-induced vascular remodeling by deubiquitinating and stabilizing SMAD7 Acta Pharmacol Sin, (2025)

PubMed: 39833306