Jagged 1 antibody

• Catalog Number: orb344625
• Category: Antibodies
• Description: Jagged 1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: This protein A purified Jagged 1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the N-terminus of human Jagged-1 protein.
• Concentration: 1.1 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:100 - 1:500, IF: 1:200 - 1:1,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This protein A purified antibody is directed against human Jagged-1 protein. The product was purified from mono-specific antiserum by affinity chromatography. A BLAST analysis was used to suggest cross reactivity with Jagged-1 protein from human, chimpanzee, rat and mouse based on 100% homology with the immunizing sequence. Partial reactivity is expected against dog (81%) and Xenopus laevis (85%) based on partial sequence homologies as indicated. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: P78504
• NCBI: 4557679
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Jagged 1 Antibody, rabbit anti-Jagged1
• Note: For research use only
• Application notes: This protein A purified antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 150 kDa in size corresponding to Jagged-1 in mouse liver, human liver and human lung whole cell lysates.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of various whole cell lysates human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only using Jagged 1 antibody