ITX3

SKU: orb2901602

Description

ITX3 is a specific and nontoxic inhibitor of TrioN (N-terminal GEF domain of the multidomain Trio protein) with an IC50 value of 76 μM. ITX3 can be used for the research of agent.

Key Properties

• CAS Number: 347323-96-0
• MW: 371.45
• Purity: >98% (HPLC)
• Formula: C₂₂H₁₇N₃OS
• SMILES: Cc1cc(\C=c2\sc3nc4ccccc4n3c2=O)c(C)n1-c1ccccc1
• Target: Rho
• Solubility: In Vitro: DMSO : 2 mg/mL (5.38 mM; Ultrasonic (<80°C)

Bioactivity

• In Vitro:
  ITX3 (5, 10, 25, 50 and 100 μM; 24 h) inhibits TrioN signaling. ITX3 (50 μM; 1 h) specifically inhibits TrioN. ITX3 (100 μM; 36 h) inhibits nerve growth factor-induced neurite outgrowth in PC12 cells. ITX3 (1, 10 and 100 μM) represses Rac1 activity and dose-dependently up-regulates the E-cadherin protein level and phospho-p38 signal in Tara-KD cells. Western blot analysis. Cell line: TrioN-, GEF337-, Tiam1- and Vav2-expressing HEK293 cell lines. Concentration: 50 μM. Incubation time: 1 hour. Result: Decreased TrioN-induced Rac activity.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Rho