ITX3

SKU: orb1707168

Description

ITX3 is a potent and selective inhibitor of the TrioN RhoGEF domain, exhibiting an IC50 of 76 µM. It effectively blocks Trio-mediated activation of RhoG and Rac1 GTPases, which underlies its utility in studying cell morphology, neurite outgrowth in PC12 cells, and fibroblast structural changes in vitro.

Research Area

Cell Biology, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 347323-96-0
• MW: 371.45
• Purity: 97.28% (May vary between batches)
• Formula: C₂₂H₁₇N₃OS
• SMILES: Cc1cc(\C=c2\sc3nc4ccccc4n3c2=O)c(C)n1-c1ccccc1
• Target: Rho,Others,ROCK
• Solubility: DMSO:4 mg/mL (10.77 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  TrioN:76 μM
• In Vitro:
  TrioN inhibitor In transfected mammalian cells, ITX3 blocked TrioN-mediated dorsal membrane ruffling and Rac1 activation while having no effect on GEF337-, Tiam1-, or Vav2-mediated RhoA or Rac1 activation. ITX3 specifically inhibited endogenous TrioN activity, as evidenced by its ability to inhibit neurite outgrowth in nerve growth factor (NGF)–stimulated PC12 cells or C2C12 differentiation into myotubes . ITX3 repressed the Rac1 activity and dose-dependently up-regulated the E-cadherin protein level in the Tara-KD cells .

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ITX 3, ITX3, ITX-3, Rho GEFs

Compound Identifiers

PubChem CID

1362865