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IRS-4 Rabbit Polyclonal Antibody

Catalog Number: orb500692

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb500692
CategoryAntibodies
DescriptionIRS-4 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityRat
ReactivityHuman, Mouse
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human IRS-4 (551-650/1257aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW134 kDa
TargetIRS4
UniProt IDO14654
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesIRS4; 160 kDa phosphotyrosine protein; Insulin rec
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NoteFor research use only
Expiration Date12 months from date of receipt.
IRS-4 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human Pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IRS-4) Polyclonal Antibody, Unconjugated (orb500692) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IRS-4 Rabbit Polyclonal Antibody

Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-IRS-4 (orb500692) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 134 kD, Observed band size: 134 kD.

IRS-4 Rabbit Polyclonal Antibody

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-IRS-4 (orb500692) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 134 kD, Observed band size: 130 kD.

  • IRS-4 Rabbit Polyclonal Antibody [orb10939]

    IF,  IHC-Fr,  IHC-P

    Mouse

    Rat

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    100 μl, 200 μl, 50 μl
  • IRS-4 Rabbit Polyclonal Antibody (HRP) [orb479355]

    IHC-Fr,  IHC-P

    Mouse

    Rat

    Rabbit

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    100 μl
  • IRS-4 Rabbit Polyclonal Antibody (AP) [orb913502]

    IHC-Fr,  IHC-P

    Mouse

    Rat

    Rabbit

    Polyclonal

    AP

    100 μl
  • IRS-4 Rabbit Polyclonal Antibody (Cy5) [orb913171]

    IF

    Mouse

    Rat

    Rabbit

    Polyclonal

    Cy5

    100 μl
  • IRS-4 Rabbit Polyclonal Antibody (APC) [orb1000320]

    IF

    Rat

    Human, Mouse

    Rabbit

    Polyclonal

    APC

    100 μl