INSM1 antibody (MSVA-456R)

• Catalog Number: orb2276423
• Category: Antibodies
• Description: INSM1 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-456R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fragment (around aa81-125) corresponding to the N-terminus of human INSM1
• Dilution range: 1:75-1:150
• Conjugation: Unconjugated
• UniProt ID: Q01101
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: A1; Insulinoma associated protein 1 (IA1); Zinc fi
• Note: For research use only
• Application notes: Positive Control: Pancreas: Islet cells should show a moderate to strong INSM1 immunostaining Negative Control: Pancreas: All acinar cells should be negative Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Moderate to strong INSM1 immunostaining in most cells of Langerhans islets of the pancreas.

Strong INSM1 immunostaining in a low grade neuroendocrine tumor of the appendix.

Strong INSM1 immunostaining of medullar but not cortical cells of the adrenal gland.