IL8 antibody

• Catalog Number: orb229133
• Category: Antibodies
• Description: Rabbit polyclonal antibody to interleukin 8 is also known as IL8/NAP1 form V and MDNCF whose expression can be detected by ER stress in a DDIT3/CHOP-dependent manner. It is found in the extracellular space. This protein has a 5-10-fold higher activity on neutrophil activation and IL-8(5-77) has increased activity on neutrophil activation along with IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200
• Conjugation: Unconjugated
• Alternative names: anti-310C antibody, anti-9E3 antibody, anti-AMCF1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Thanh-Tam Tran, Gyuseok Lee, Yun Hyun Huh, Ki-Ho Chung, Sun Young Lee, Ka Hyon Park, Seung Hee Kwon, Min-Suk Kook, Jang-Soo Chun, Jeong-Tae Koh, Je-Hwang Ryu Disruption of cholesterol homeostasis triggers periodontal inflammation and alveolar bone loss Exp Mol Med, (2023)

PubMed: 38036731

Applications

IHC

Reactivity

Human

He, Yong et al. Major depression accompanied with inflammation and multiple cytokines alterations: Evidences from clinical patients to macaca fascicularis and LPS-induced depressive mice model J Affect Disord, 271, 262-271 (2020)

PubMed: 32479325

Applications

WB

Reactivity

Mouse

Western blot analysis of extracts from human liver using IL8 antibody.

Effect of JHQ on core target proteins abundant in LX-2 cells after CuSO4 treatment. ∗∗∗P < 0.001 and ∗∗P < 0.01 compared with the model group.

Efects of inhibition of HIF-1α on expression of infammation after hyperglycemic and hypoxia.b Cells were treated with KC7F2 (10 µM) or si-HIF-1α for 48 h following combined stimulation, and IL-6, IL-8, ICAM-1, MCP-1, and c HIF-1α levels were analyzed. The

Effects of inhibition of HIF-1α on expression of inflammation after hyperglycemic and hypoxia. a Cells were treated with glucose (25 mM) or with combined exposure to high glucose and hypoxia for 6, 12, 24, and 48 h. Exposure to glucose alone or combined s

Prostatic fluid exosomes promoted inflammation in prostate tissue. (A) Representative HE-staining images of prostate tissue in SD rats injected with normal saline, NC exosomes, prostatic fluid exosomes of CP/CPPS-A patients, and ultrasound-treated prostat

FGF21 attenuates the expression of NF-κB, Iba1, IL6 and IL8 in the brains of diabetic mice (n = 10/group). (A)The IL6 levels of each group mice were exhibited by immunohistochemistry (40X magnification, bar = 20 μm). (B) The levels of NF-κB, Iba1, IL6 and

FGF21 attenuates neurodegeneration through anti-inflammation in aging mice (n = 7-8/group). (D) The injury levels were exhibited by H&E staining (10X magnification, bar = 100 μm) and IL6, IL8 levels were exhibited by immunohistochemistry (40X magnificatio

The inhibitory effect of BML-111 on the tumor growth of 4T1-MIND model mice in vivo (A) Representative images of the tumor tissue dissected from 4T1-MIND model mice. *P = 0.0271 BML-111 group vs. control group. (F) The expression of IL-8 in tumor cells an

Expression levels of CXCL8 in the clinical cohort. (A) The levels of CXCL8 mRNA in the normal (N = 81), colorectal cancer (CRC)-early stage (N = 44)and CRC-advanced stage (N = 37) groups. (B) The levels of CXCL8 protein in normal (N = 87), CRC-early stage

qRT-PCR and western blot results. (A, B) qRT-PCR results showed that CD274 and IL8 were upregulated in the basal-like breast cancer cell lines BT549 and MDA-MB-231 (p < 0.0001). Similar to the qRT-PCR results, the western blot analysis (C–E) indicated tha

PRL‑3‑induced activation of IL‑6 and IL‑8 by initiating JNK and ERK pathways in TAMs. (A) After co‑culture with colorectal cancer cells, IL‑6, IL‑8, p‑JNK, and p‑ERK levels in TAMs were examined using western blot assays.

Immunohistochemical staining of a) IL-8 and b) MCP-1 in the wound bed on day 3, 7, and 14. Scale bars represent 100 µm. c) Semiquantitative analysis of IL-8 and MCP-1, double-blind analysis by using a four-point scale (0, no positive cells; 1, low number