IL12_2 Antibody (C-term)

• Catalog Number: orb1938791
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB28530
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 97135 Da
• Target: This IL12_2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 756-783 amino acids from the C-terminal region of human IL12_2.
• UniProt ID: Q99665
• NCBI: NP_001245143.1, NP_001245144.1, NP_001550.1, NP_001245145.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Interleukin-12 receptor subunit beta-2, IL-12 rece
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-IL12RB2 Antibody (C-term) at 1:2000 dilution. Lane 1: THP-1 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: MOLT-4 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 97 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

IL12RB2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsils tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL12RB2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

IL12RB2 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35 ug/lane).

Overlay histogram showing A431 cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.