IL-8RA Antibody [SE2], Rabbit IgG

• Catalog Number: orb1671592
• Category: Antibodies
• Description: IL-8RA Antibody [SE2], Rabbit IgG
• Clonality: Recombinant
• Clone Number: SE2
• Tested applications: FC, IHC, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunizing BALB/c mice with a BSA-conjugated peptide comprising of the N-terminal part of IL-8RA (amino acids 1-30).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: CXCR1
• UniProt ID: P25024
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CXCR-1, C-X-C chemokine receptor type 1, CXC-R1, C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-IL-8RA antibody SE2. Human lymph node tissue lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SE2 (orb1671592) at 0.1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-IL-8RA antibody SE2. Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix™ coverslips stained with the chimeric r version of SE2 (orb1671592) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671592- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the Anti-IL-8RA antibody SE2 (Paraformaldehyde fixed human peripheral blood monocytes were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of SE2 (orb1671592 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.