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Catalog Number | orb1934352 |
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Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB3263 |
Tested applications | FC, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 21893 Da |
Target | This IL-27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-87 amino acids from the Central region of human IL-27. |
UniProt ID | Q8TAD2 |
NCBI | NP_612141.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Interleukin-17D, IL-17D, Interleukin-27, IL-27, IL Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of IL27 (arrow) using rabbit polyclonal IL27 Antibody (C71). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IL27 gene.
IL-27 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IL-27 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of IL-27 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.