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IGF2 Antibody (Center R54)

Catalog Number: orb1938352

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1938352
CategoryAntibodies
DescriptionAffinity Purified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB18750
Tested applicationsFC, IHC-P, WB
ReactivityHuman, Mouse
IsotypeRabbit IgG
Antibody TypePrimary Antibody
Dilution rangeWB: 1:2000, WB: 1:2000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:25
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
ConjugationUnconjugated
MW20140 Da
TargetThis IGF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the Central region of human IGF2.
UniProt IDP01344
NCBINP_001121070.1, NP_001007140.2, NP_000603.1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesInsulin-like growth factor II, IGF-II, Somatomedin
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NoteFor research use only
Expiration Date12 months from date of receipt.
IGF2 Antibody (Center R54)

All lanes: Anti-IGF2 Antibody (Center R54) at 1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 20 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

IGF2 Antibody (Center R54)

Anti-IGF2 Antibody (Center R54) at 1:2000 dilution + mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 20 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

IGF2 Antibody (Center R54)

IGF2 Antibody (Center R54) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of IGF2 Antibody (Center R54) for immunohistochemistry. Clinical relevance has not been evaluated.

IGF2 Antibody (Center R54)

IGF2 Antibody (Center R54) western blot analysis in mouse cerebellum tissue lysates (35 ug/lane). This demonstrates the IGF2 antibody detected the IGF2 protein (arrow).

IGF2 Antibody (Center R54)

Overlay histogram showing Hela cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

  • IGF2 Antibody (Center R54) [orb1168070]

    FC,  IHC-P,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 30 μl