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IFNAR2 Rabbit Polyclonal Antibody
Description
Images & Validation
−| Tested Applications | ELISA, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200, ELISA=1:1000-5000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from mouse IFNAR2 (165-260/513aa) |
| Target | IFNAR2 |
| Molecular Weight | 54 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
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50 μl, 100 μlIFNAR2 Antibody [orb627930]
ELISA, IF, WB
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Rabbit
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100 μg, 50 μg

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Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-IFNAR2/IFNABR Polyclonal Antibody, Unconjugated (orb100572) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
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Protocol Information
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Hayashi, Yushi et al. Cochlear supporting cells function as macrophage-like cells and protect audiosensory receptor hair cells from pathogens Sci Rep, 10, 6740 (2020)
Applications
Reactivity
IFNAR2 Rabbit Polyclonal Antibody (orb100572)
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