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IDS/Iduronate 2 Sulfatase Antibody

SKU: orb1539144

Description

IDS/Iduronate 2 Sulfatase Antibody

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P, WB
Dilution RangeIHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:16000), WB (0.1 - 0.3 µg/ml)
ReactivityHuman, Monkey
Application Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx. 60kDa band observed in Human Liver lysates (calculated MW of 61.9kDa according to NP_000193.1). Recommended concentration: 0.1-0.3 ug/ml.

Key Properties

HostGoat
ClonalityPolyclonal
ImmunogenPeptide with sequence C-KHFRFRDLEEDP, from the internal region of the protein sequence according to NP_000193.1.
TargetIDS / Iduronate 2 Sulfatase
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration0.5 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

IDS, SIDS, Iduronate 2-sulfatase, MPS2

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IDS/Iduronate 2 Sulfatase Antibody

IDS antibody (0.1 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

IDS/Iduronate 2 Sulfatase Antibody (orb1539144)

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Available Sizes

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50 μg
$ 670.00
DispatchUsually dispatched within 2-3 weeks
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