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Catalog Number | orb1249287 |
---|---|
Category | Antibodies |
Description | IDH3G Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Bovine, Canine, Mouse |
Reactivity | Human, Porcine, Rat |
Immunogen | The immunogen for this antibody is: C-DHLKLHSYATSIRK |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 38kDa band observed in lysates of cell line MCF7 and of Rat and Pig Heart (calculated MW of 41.5kDa according to NP_777358.1). Recommended concentration: 0.5-2ug/ml.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | IDH3G |
UniProt ID | P51553 |
NCBI | NP_777358.1, NP_004126.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | IDH3G, isocitrate dehydrogenase 3 (NAD+) gamma, H- Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 38kDa band observed in lysates of cell line MCF7 and of Rat and Pig Heart (calculated MW of 41.5kDa according to NP_777358.1). Recommended concentration: 0.5-2ug/ml.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1249287 (0.5 ug/ml) staining of MCF7 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1249287 (1 ug/ml) staining of Rat (A) and Pig (B) Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1249287 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
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