IDH3G Antibody

SKU: orb1249287

Description

IDH3G Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Porcine, Rat
• Predicted Reactivity: Bovine, Canine, Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 38kDa band observed in lysates of cell line MCF7 and of Rat and Pig Heart (calculated MW of 41.5kDa according to NP_777358.1). Recommended concentration: 0.5-2ug/ml.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DHLKLHSYATSIRK
• Target: IDH3G
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

IDH3G, isocitrate dehydrogenase 3 (NAD+) gamma, H-IDHG, IDH-gamma, NAD (H)-specific isocitrate dehydrogenase gamma subunit, NAD(+)-specific ICDH subunit gamma, NAD+-specific ICDH, OTTHUMP00000025984, OTTHUMP00000025987, OTTHUMP00000025988, isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, gamma subunit, isocitric dehydrogenase subunit gamma

orb1249287 (0.5 ug/ml) staining of MCF7 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249287 (1 ug/ml) staining of Rat (A) and Pig (B) Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249287 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

IDH3G

UniProt

P51553

RefSeq (Protein):NP_004126.1, NP_777358.1