IDH3B Antibody

• Catalog Number: orb1249286
• Category: Antibodies
• Description: IDH3B Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Canine
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: The immunogen for this antibody is: C-HAASRSQAEDVRVE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 39kDa band observed in Mouse, Rat and Pig Heart lysates and in Human and Mouse Skeletal Muscle lysates (calculated MW of 41.9kDa according to NP_777280.1). Recommended concentration: 0.3-1ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: IDH3B
• UniProt ID: O43837
• NCBI: NP_008830.2, NP_777280.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: IDH3B, isocitrate dehydrogenase 3 (NAD+) beta, FLJ
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 39kDa band observed in Mouse, Rat and Pig Heart lysates and in Human and Mouse Skeletal Muscle lysates (calculated MW of 41.9kDa according to NP_777280.1). Recommended concentration: 0.3-1ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249286 (0.3 ug/ml) staining of Human Skeletal muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249286 (2 ug/ml) staining of Mouse Heart (A) and Mouse Muscle (B) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249286 (0.1 ug/ml) staining of Pig Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.