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IDH3B Antibody

SKU: orb1249286

Description

IDH3B Antibody

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman, Mouse, Porcine, Rat
Predicted ReactivityCanine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 39kDa band observed in Mouse, Rat and Pig Heart lysates and in Human and Mouse Skeletal Muscle lysates (calculated MW of 41.9kDa according to NP_777280.1). Recommended concentration: 0.3-1µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-HAASRSQAEDVRVE
TargetIDH3B
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

IDH3B, isocitrate dehydrogenase 3 (NAD+) beta, FLJ11043, H-IDHB, MGC903, RP46, NAD(+)-specific ICDH subunit beta, NAD+-specific ICDH, NAD+-specific isocitrate dehydrogenase b subunit, NAD+-specific isocitrate dehydrogenase beta, isocitrate dehydrogenase [NAD] subunit beta, mitochondrial, isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, beta subunit, isocitric dehydrogenase subunit beta

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IDH3B Antibody

orb1249286 (0.3 ug/ml) staining of Human Skeletal muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

IDH3B Antibody

orb1249286 (2 ug/ml) staining of Mouse Heart (A) and Mouse Muscle (B) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

IDH3B Antibody

orb1249286 (0.1 ug/ml) staining of Pig Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

IDH3B Antibody (orb1249286)

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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