HTT Antibody

SKU: orb345421

Description

Huntington (phospho-S421) antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:10,000 - 1:40,000, IHC: 1:50 - 1:100, WB: 1:500 - 1:3,000
• Reactivity: Human
• Application Notes:
  Anti-Huntingtin pS421 antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect bands at approximately 350 kDa and 200 kDa in size corresponding to full-length Huntingtin protein and truncated (hypothetical) Huntingtin protein, respectively, by western blotting in the appropriate cell lysate or extract. This antibody is specific for the phosphorylated form of Huntingtin protein at the pS421 residue. The identity of lower molecular bands ~130 kDa is not known.

  Read more...

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Huntingtin pS421 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 400-425 of Human Huntington Disease Protein.
• Target: HTT
• Purity: Anti-Huntingtin pS421 is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human Huntington protein at the pS421 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Huntington homologues from chimpanzee, pig and chicken. Cross reactivity with Huntington protein homologues from mouse and rat may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Huntington protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.96 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Huntington pS421 antibody, rabbit anti-Huntingtin pS421 antibody, HD protein, HTT, Huntington Disease Protein, Huntington disease, IT15, LOMARS

Quick Database Links

Gene Symbol

HTT

UniProt

P42858

RefSeq (Protein):NP_002102.4