HTT Antibody

SKU: orb345749

Description

Huntington antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:60,000, IHC: 1:100-1:500, IF: 1:100-1:500, WB: 0.1 µg/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Anti-Huntingtin antibody has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~350 kDa corresponding to the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Huntington affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the near N-terminus of human Huntington disease protein.
• Target: HTT
• Purity: Anti-Huntingtin was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards HTT. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, and Rat based on 100% sequence homology. Cross-reactivity with HTT from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: None. Stabilizer: 50% (v/v) Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Huntington antibody, rabbit anti-Huntingtin antibody, HD protein, HTT, Huntington Disease Protein, Huntington disease, IT15, LOMARS

Western Blot of Rabbit Anti-Huntington antibody. Lane 1: mouse brain extract absence of blocking peptide. Lane 2: mouse brain extract with blocking peptide. Load: 10 µg per lane. Primary antibody: Huntington antibody at 0.1 µg/ml for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 350 kDa for Huntington. Other band(s): Huntington splice variants and isoforms.

Quick Database Links

Gene Symbol

HTT

UniProt

P42858

RefSeq (Protein):NP_002102.4