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Catalog Number | orb392639 |
---|---|
Category | Proteins |
Description | Recombinant human Transthyretin protein is produced by our Mammalian expression system and the target gene encoding Gly21-Glu147 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | TTR |
Entrez | 7276 |
UniProt ID | P02766 |
Protein Sequence | GPTGTGESKC PLMVKVLDAV RGSPAINVAV HVFRKAADDT WEPFASGKTS ESGELHGLTT EEEFVEGIYK VEIDTKSYWK ALGISPFHEH AEVVFTANDS GPRRYTIAAL LSPYSYSTTA VVTNPKEVDH HHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. |
Alternative names | PALB; Transthyretin; ATTR; Prealbumin; TBPA Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE, WB | |
Unconjugated | |
> 85% as determined by SDS-PAGE | |
21 kDa |
Unconjugated | |
95% | |
14.9 kDa | |
Human Transthyretin, His Tag (orb257905) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Glu 147 (Accession # NP_000362). |
Greater than 95% as determined by SDS-PAGE. | |
43.9 kDa | |
Mammalian cell |
Greater than 90% as determined by SDS-PAGE. | |
17.8 kDa | |
E.coli |
Human | |
1.56 μg/mL-25 μg/mL | |
0.78 μg/mL |
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