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Catalog Number | orb707067 |
---|---|
Category | Proteins |
Description | Recombinant Human Tryptase alpha/beta Protein is produced by mammalian expression system and the target gene encoding Ile31-Pro275 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | TPSAB1 |
Entrez | 7177 |
UniProt ID | Q15661 |
Protein Sequence | IVGGQEAPRS KWPWQVSLRV RDRYWMHFCG GSLIHPQWVL TAAHCLGPDV KDLATLRVQL REQHLYYQDQ LLPVSRIIVH PQFYIIQTGA DIALLELEEP VNISSRVHTV MLPPASETFP PGMPCWVTGW GDVDNDEPLP PPFPLKQVKV PIMENHICDA KYHLGAYTGD DVRIIRDDML CAGNTRRDSC QGDSGGPLVC KVNGTWLQAG VVSWDEGCAQ PNRPGIYTRV TYYLDWIHHY VPKKPVDHHH HHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | TPS1 protein, TPS2 protein, TPSB1 protein, Tryptas Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 29.6 kDa after removal of the signal peptide. The apparent molecular mass of TPSAB1-His is approximately 35-40 kDa due to glycosylation. | |
Mammalian |
98.00% | |
32-38 KDa (reducing condition) |
Greater than 90.0% as determined by SDS-PAGE. | |
Sf9, Baculovirus cells |
Greater than 85% as determined by SDS-PAGE. | |
E.coli |